Proteomics-based Expression Library Screening (PELS)
نویسندگان
چکیده
منابع مشابه
Library screening by fragment-based docking.
We review our computational tools for high-throughput screening by fragment-based docking of large collections of small molecules. Applications to six different enzymes, four proteases, and two protein kinases, are presented. Remarkably, several low-micromolar inhibitors were discovered in each of the high-throughput docking campaigns. Probable reasons for the lack of submicromolar inhibitors a...
متن کاملLibrary design for fragment based screening.
According to Hann's model of molecular complexity an increased probability of detection binding to a target protein can be expected when small, low complex molecular fragments are screened with high sensitivity instead of full-sized ligands with lower sensitivity. Analysis of the HTS summary data of Novartis and comparison with NMR screening results obtained on generic fragment libraries indica...
متن کاملCoESPRIT: A Library-Based Construct Screening Method for Identification and Expression of Soluble Protein Complexes
Structural and biophysical studies of protein complexes require multi-milligram quantities of soluble material. Subunits are often unstable when expressed separately so co-expression strategies are commonly employed since in vivo complex formation can provide stabilising effects. Defining constructs for subunit co-expression experiments is difficult if the proteins are poorly understood. Even m...
متن کاملgel-based proteomics
proteomics has emerged since 1995 for studying the total proteome of cells, tissues, or organisms at any condition. the study of proteins has a fundamental importance because they are the functional and final molecules of the gene expression process and proteomics could be a valuable technique for achieving this purpose. mass spectrometry is a complementary technique for proteomics that allows ...
متن کاملApproaches to library screening.
Fuelled by the drive to complete the Human Genome Project, many laboratories have developed new methods of screening clone libraries. From PCR-based strategies to pooling schemes and increased automation, the tedious task of library screening has become less labour-intensive and more cost-efficient. Currently, two main screening methods dominate: hybridization and polymerase chain reaction (PCR...
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2006
ISSN: 1535-9476
DOI: 10.1074/mcp.t600013-mcp200